PHYTOCHEMICAL AND ANTIVENOM STUDIES ON THE STEM BARK OF ALBIZIA CHEVALIERI HAMS (MIMOSACEAE) ON NAJA NIGRICOLLIS BROADLEY ENVENOMATED MICE
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
ABSTRACT
Albizia chevalieri Hams (Mimosaceae), mostly found in the Northern Sahel Savannah region of Nigeria as well as in Nigér and Senegal is a tree of the acacia type with a long list of folklore therapeutic claims which include its use as purgative, taenicidal, cough remedy, dysentery, cancer, diabetes mellitus, tuberculosis and snake bite remedy. The stem bark was collected, dried and powdered. The powder was firstly extracted with hexane, and subsequently with methanol. The methanolic extract was then fractionated into ethyl acetate (EtOAc) and n-butanol soluble parts. Chromatographic separation of the EtOAc and hexane fractions led to the isolation of four pure compounds—β-sitosterol (EAC1) and three pentacyclic triterpenoids (Friedelin, Friedelinol and Lupeol, labelled as HXC1, HXC2, and HXC3 respectively), which were characterized using spectroscopic techniques (HREIMS, NMR, IR and UV). Two of the four isolated compounds (HXC1 and EAC1), were investigated for antivenom activity, including the extract and fractions in experimental albino mice using standard methods. The results showed that the isolated compound, EAC1 offered the highest protection to the animals by prolonging time of death of animal in the treated group at a dose dependant concentration of 8mgkg-1 with a minimum survival rate of 60%, followed by compound HXC1 which recorded 20% survival rate at same doses; compared to the group administered with the minimum lethal dose (MLD) of venom only, where 80% of the animals died within an average time of 17minutes. The fractions of n-butanol and hexane recoded antivenom activity at 200 mgkg-1 while those of methanol and EtOAc recorded no activity. Gross histopathological changes in liver and kidney tissues of envenomated mice treated with two of the isolated compounds were observed to be less obvious than seen in the tissues of envenomated mice treated with the candidate plant extracts and fractions. This study went to show the likely antivenom potentials in the isolated compounds evaluated as well as those of the fractions of n-butanol and hexane, which is being reported for the first viii time in the plant A. chevalieri. The results obtained also provided scientific basis for the use of A. chevalieri in folklore medicine in the treatment of snake bites.
Related Project Materials
THE IMPACT OF GLOBALIZATION ON VOCATIONAL CURRICULUM DEVELOPMENT
Abstract: This study investigates the impact of globalization on vocational curriculum deve...
THE IMPACT OF COSTING TECHNIQUES ON PROFITABILITY OF A MANUFACTURING COMPANY
ABSTRACT
This study sought to analyze costing techniques and their impact on profitability of manufacturing companies....
PARENTING STYLES AND SELF CONCEPT ON EMOTIONAL ADJUSTMENT OF NIGERIA SECONDARY SCHOOL STUDENTS IN IKPOBA OKHA LOCAL GOVERNMENT AREA OF EDO STATE
ABSTRACT
This study investigates parenting styles and self concept. It was wi...
ROLES OF SELF OBJECTIFICATION AND APPEARANCE ANXIETY ON MARITAL SATISFACTION
ABSTRACT
This study investigated the role of self-objectification and Appearance Anxiety on marital satisfaction among...
THE IMPACT OF SALES FORECASTING ON BUSINESS PLANNING
THE IMPACT OF SALES FORECASTING ON BUSINESS PLANNING
This study examines the impact of...
ASSESSING THE USE OF TRADITIONAL MEDICINE IN THE TREATMENT OF MALARIA AMONG PREGNANT WOMEN
BACKGROUND OF TJHE STUDY
Malaria is a potentially fatal parasite illness spread by female Anopheles mos...
INNOVATIONS IN ASSESSING TEAMWORK AND COLLABORATION IN VOCATIONAL SETTINGS
Abstract: Innovations in assessing teamwork and collaboration in vocational settings are es...
RECYCLING AND REUSE: ALTERNATIVES TO WASTE MANAGEMENT IN NIGERIA
Abstract
Waste is a global environmental issue that is becoming most noted in developing countries. Pu...
THE ROLES OF AUDITORS AND METHOD OF INTERNAL CONTROL IN LOCAL GOVERNMENT
ABSTRACT
The subject of this research investigation is “The Role of Internal Auditor and the method of Internal Co...
THE CHALLENGES FACED BY COMMERCIAL VEHICLES IN ABUJA
EXCERPT FROM THE STUDY
The Federal Road Safety Corps (FRSC, 2010) in its research monograph "Road Mirror‟ found tha...
